Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK 293FLP
genotype/variation
WT
antibody
H3K4me3
antibody vendor
abcam
antibody catalog
ab8580

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA has been isolated with the PureLink Genomic DNA kit (Thermo Fisher Scientific), according to manufacturer's instructions. Libraries have been build with the TruSeq ChIP Library Prep Kit, according to manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
20767696
Reads aligned (%)
90.6
Duplicates removed (%)
90.3
Number of peaks
1581 (qval < 1E-05)

hg19

Number of total reads
20767696
Reads aligned (%)
90.3
Duplicates removed (%)
90.5
Number of peaks
1600 (qval < 1E-05)

Base call quality data from DBCLS SRA