GSM2299065: Sequencing analysis of input in mouse myotubes under shPhf5a knockdown treatment; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Muscle
Cell type
Myotube
NA
NA
Attributes by original data submitter
Sample
source_name
Myotubes shPhf5a
cell type
mouse myotubes
chip antibody
Input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ten million cells were used for the ChIP and precipitated using 5 micrograms of respective antibodies. Original reference: Zhu B. et al. (2005) "The human PAF complex coordinates transcription with events downstream of RNA synthesis" Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.