Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Muscle

Cell type

Myoblasts

MeSH Description

Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE).

Sample

source_name

Myoblasts shControl

cell type

mouse myoblasts

chip antibody

Input

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Ten million cells were used for the ChIP and precipitated using 5 micrograms of respective antibodies. Original reference: Zhu B. et al. (2005) "The human PAF complex coordinates transcription with events downstream of RNA synthesis" Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

31928735

Reads aligned (%)

97.8

Duplicates removed (%)

13.2

Number of peaks

493 (qval < 1E-05)

mm9

Number of total reads

31928735

Reads aligned (%)

97.5

Duplicates removed (%)

13.2

Number of peaks

518 (qval < 1E-05)