Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Th1 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Sample

source_name

Th1 cells

cell type

primary mouse T cells

culture

Th1 condition for 3 days

background strain

C57BL/6

genotype/variation

Wild-type

antibody

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitations were performed with SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) according to manufacturer’s instructions. DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

12865675

Reads aligned (%)

96.3

Duplicates removed (%)

1.2

Number of peaks

120 (qval < 1E-05)

mm9

Number of total reads

12865675

Reads aligned (%)

96.2

Duplicates removed (%)

1.3

Number of peaks

87 (qval < 1E-05)