Curated Sample Data


Genome
dm3
Antigen Class
Histone
Antigen
H3
Cell type Class
Cell line
Cell type
S2-DRSC

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
H3_CtrlRNAi, Drosophila S2 cells
cell line
S2-DRSC
treatment
Ctrl RNAi
chip antibody
rabbit anti-H3 (Abcam Cat# ab1791 RRID:AB302613)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For chromatin immunoprecipitation (ChIP) cells were crosslinked with 1 % formaldehyde for 5 min at room temperature. Upon cell lysis, protease inhibitors and proteasome inhibitor MG-132 (Enzo Life Sciences) were applied. The chromatin was isolated and sheared with adaptive focused acoustics (Covaris) to an average size of 200 base pair (bp). For each ChIP reaction, chromatin isolated from 1-2 x 10e6 cells was incubated with antibodies precoupled to Protein A/G Sepharose. All libraries were prepared using MicroPlex (Diagenode) or NEBNext (NEB) Library Preparation kit.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
21646028
Reads aligned (%)
96.0
Duplicates removed (%)
13.2
Number of peaks
3736 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA