Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Hmr

Cell type

Cell type Class
Cell line
Cell type
S2-DRSC
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
HMR, Drosophila S2 cells
cell line
S2-DRSC
treatment
untreated
chip antibody
rat anti-HMR 2C10 (RRID: AB2569849)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For chromatin immunoprecipitation (ChIP) cells were crosslinked with 1 % formaldehyde for 5 min at room temperature. Upon cell lysis, protease inhibitors and proteasome inhibitor MG-132 (Enzo Life Sciences) were applied. The chromatin was isolated and sheared with adaptive focused acoustics (Covaris) to an average size of 200 base pair (bp). For each ChIP reaction, chromatin isolated from 1-2 x 10e6 cells was incubated with antibodies precoupled to Protein A/G Sepharose. All libraries were prepared using MicroPlex (Diagenode) or NEBNext (NEB) Library Preparation kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
22695222
Reads aligned (%)
71.3
Duplicates removed (%)
18.2
Number of peaks
6178 (qval < 1E-05)

dm6

Number of total reads
22695222
Reads aligned (%)
66.2
Duplicates removed (%)
23.4
Number of peaks
3868 (qval < 1E-05)

Base call quality data from DBCLS SRA