Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Kidney
Cell type
Renal cell carcinoma
NA
NA

Attributes by original data submitter

Sample

source_name
ccRCC cell line
histone
H3K4me1
chip antibody
H3K4me1 (ab8895, Abcam)
source
in house derived

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
One million cells were fixed in 1% formaldehyde/PBS buffer for 10 min at room temperature. Fixation was stopped by addition of glycine to a final concentration of 125 mM. Cells were washed 3 times with TBSE buffer. Pelleted cells were lysed in 100 µl 1% SDS lysis buffer and sonicated to 300-500bp using a Bioruptor (Diagenode). The total volume of immunoprecipitation was 1 ml and the amount of antibody used was 2 µg. The input DNA was precleared with protein G Dynabeads (Life Technologies) for 1 hr at 4°C and then incubated with antibodies conjugated protein G beads overnight at 4°C. The beads were washed 3 times with cold wash buffer. Whole-genome-amplification was performed on ChIP DNA and input using the WGA4 kit (Sigma-Aldrich). The amplified DNA was used with NEBNext ChIP-Seq library prep reagent set.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
29442478
Reads aligned (%)
93.2
Duplicates removed (%)
24.1
Number of peaks
28818 (qval < 1E-05)

hg19

Number of total reads
29442478
Reads aligned (%)
92.9
Duplicates removed (%)
24.4
Number of peaks
28723 (qval < 1E-05)

Base call quality data from DBCLS SRA