Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

ESC_WT

strain background

D3

cell type

Embryonic stem cells

genotype/variation

WT

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were sonicated to fragment chromatin, pre-cleared by centrifugation, and subjected to immunoprecipitation with CBX2 antibody. Libraries were prepared as described in PMID: 23837789.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

35786940

Reads aligned (%)

98.5

Duplicates removed (%)

9.1

Number of peaks

207 (qval < 1E-05)

mm9

Number of total reads

35786940

Reads aligned (%)

98.3

Duplicates removed (%)

9.9

Number of peaks

198 (qval < 1E-05)