Antigen

Antigen Class

TFs and others

Antigen

EP300

Cell type

Cell type Class

Blood

Cell type

RCH-ACV

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

Leukemia cell line (RCH-ACV)

cell type

Paediatric pre B-cell line derived from cALL with t(1;19)(q23;p13) translocation

chip antibody

p300 (Bethyl, A300-358A/3)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

108 fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the NEBNext DNA (Part# E6040) or Ultra RNA (Part# E7530) Library Prep Master Mix Set.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg19

Number of total reads

31093245

Reads aligned (%)

88.4

Duplicates removed (%)

49.1

Number of peaks

21779 (qval < 1E-05)

hg38

Number of total reads

31093245

Reads aligned (%)

90.1

Duplicates removed (%)

48.6

Number of peaks

21946 (qval < 1E-05)