GSM2290172: RCH P300 ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EP300
Cell type
Cell type Class
Blood
Cell type
RCH-ACV
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
Leukemia cell line (RCH-ACV)
cell type
Paediatric pre B-cell line derived from cALL with t(1;19)(q23;p13) translocation
chip antibody
p300 (Bethyl, A300-358A/3)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
108 fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the NEBNext DNA (Part# E6040) or Ultra RNA (Part# E7530) Library Prep Master Mix Set.