Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Blood
Cell type
RCH-ACV
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
Leukemia cell line (RCH-ACV)
cell type
Paediatric pre B-cell line derived from cALL with t(1;19)(q23;p13) translocation
chip antibody
p300 (Bethyl, A300-358A/3)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
108 fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the NEBNext DNA (Part# E6040) or Ultra RNA (Part# E7530) Library Prep Master Mix Set.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
31093245
Reads aligned (%)
88.4
Duplicates removed (%)
49.1
Number of peaks
21779 (qval < 1E-05)

hg38

Number of total reads
31093245
Reads aligned (%)
90.1
Duplicates removed (%)
48.6
Number of peaks
21946 (qval < 1E-05)

Base call quality data from DBCLS SRA