Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF3

Cell type

Cell type Class
Blood
Cell type
SEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
Leukemia cell line (SEM)
cell type
Paediatric pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation
chip antibody
E2A (Cell Signaling, 12258/1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
108 fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the NEBNext DNA (Part# E6040) or Ultra RNA (Part# E7530) Library Prep Master Mix Set.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
40830683
Reads aligned (%)
164.5
Duplicates removed (%)
11.1
Number of peaks
14601 (qval < 1E-05)

hg38

Number of total reads
40830683
Reads aligned (%)
166.5
Duplicates removed (%)
10.8
Number of peaks
14911 (qval < 1E-05)

Base call quality data from DBCLS SRA