Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Neural
Cell type
Glioma tumorspheres
NA
NA

Attributes by original data submitter

Sample

source_name
H3K4me3 TS603
cell type
glioma tumorsphere
genotype
IDH1 R132H
passage
N/A
chip antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the Magna ChIP™ G - Chromatin Immunoprecipitation Kit (Millipore) according to manufacturer's instructions. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
25971019
Reads aligned (%)
63.7
Duplicates removed (%)
1.6
Number of peaks
23446 (qval < 1E-05)

hg19

Number of total reads
25971019
Reads aligned (%)
62.8
Duplicates removed (%)
1.7
Number of peaks
23325 (qval < 1E-05)

Base call quality data from DBCLS SRA