Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Astrocytes
MeSH Description
A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.

Attributes by original data submitter

Sample

source_name
INPUT PAR P10
cell type
immortalized human astrocytes
genotype
parental
passage
10
chip antibody
INPUT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the Magna ChIP™ G - Chromatin Immunoprecipitation Kit (Millipore) according to manufacturer's instructions. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
26781504
Reads aligned (%)
61.0
Duplicates removed (%)
1.2
Number of peaks
459 (qval < 1E-05)

hg19

Number of total reads
26781504
Reads aligned (%)
59.9
Duplicates removed (%)
1.3
Number of peaks
375 (qval < 1E-05)

Base call quality data from DBCLS SRA