GSM2286774: MIA PACa-2 siMLL1 IP H3K4me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pancreas
Cell type
MIA Paca-2
Primary Tissue
Pancreas
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
epithelial cells derivef from pancreatic carcinoma
cell line
MIA PaCa-2 pancreatic cancer cell line
antibody
Anti-Histone H3 (tri-methyl K4)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation and final DNA purification was carried out using the SimpleChIP Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology) NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® (E6240, New England BioLabs)