Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pancreas
Cell type
MIA Paca-2
Primary Tissue
Pancreas
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
epithelial cells derivef from pancreatic carcinoma
cell line
MIA PaCa-2 pancreatic cancer cell line
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation and final DNA purification was carried out using the SimpleChIP Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology) NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® (E6240, New England BioLabs)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
40269478
Reads aligned (%)
96.7
Duplicates removed (%)
10.3
Number of peaks
1186 (qval < 1E-05)

hg19

Number of total reads
40269478
Reads aligned (%)
95.5
Duplicates removed (%)
12.8
Number of peaks
949 (qval < 1E-05)

Base call quality data from DBCLS SRA