Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Adult

Cell type

Whole worm

NA

NA

Sample

source_name

WT_NT_H3K4me3

developmental stage

L3-L4 mix

treated with

none; no treatment (NT)

tissue

whole body

chip antibody

H3K4me3 (Abcam, #ab8580)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Worms were collected when became L3-L4 mix and handled with standard ChIP protocol. Purified DNA was used for DNA library construction using NEBNext Ultra DNA Library Prep Kit for Illumina.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

ce11

Number of total reads

6426854

Reads aligned (%)

79.5

Duplicates removed (%)

6.5

Number of peaks

3158 (qval < 1E-05)

ce10

Number of total reads

6426854

Reads aligned (%)

79.5

Duplicates removed (%)

6.5

Number of peaks

3160 (qval < 1E-05)