Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Adult
Cell type
Whole worm
NA
NA

Attributes by original data submitter

Sample

source_name
WT_NT_H3K4me3
developmental stage
L3-L4 mix
treated with
none; no treatment (NT)
tissue
whole body
chip antibody
H3K4me3 (Abcam, #ab8580)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Worms were collected when became L3-L4 mix and handled with standard ChIP protocol. Purified DNA was used for DNA library construction using NEBNext Ultra DNA Library Prep Kit for Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce11

Number of total reads
6460224
Reads aligned (%)
81.4
Duplicates removed (%)
7.2
Number of peaks
3127 (qval < 1E-05)

ce10

Number of total reads
6460224
Reads aligned (%)
81.4
Duplicates removed (%)
7.2
Number of peaks
3132 (qval < 1E-05)

Base call quality data from DBCLS SRA