Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Medial ganglionic eminence
NA
NA

Attributes by original data submitter

Sample

source_name
Medial ganglionic eminence
strain
C57BL/6
chip antibody
None
genotype
Nkx2-1cKO
age
embryonic day 13.5
tissue
Medial ganglionic eminence

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was micro-dissected and prepared for TF or native ChIP, cross-linked, and sheared. Protein/antibody complexes were isolated with antibodies and collected using Dynabeads. ChIP-seq libraries were generated using the Ovation Ultralow DR Multiplex System (NuGEN #0330).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
23526385
Reads aligned (%)
98.2
Duplicates removed (%)
12.0
Number of peaks
206 (qval < 1E-05)

mm9

Number of total reads
23526385
Reads aligned (%)
98.0
Duplicates removed (%)
12.0
Number of peaks
207 (qval < 1E-05)

Base call quality data from DBCLS SRA