Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
TL-Om1
NA
NA

Attributes by original data submitter

Sample

source_name
TL-Om1 cells
cell line
TL-Om1
cell type
adult T-cell leukemia/lymphoma (ATL) cell line
treated with
control DMSO for 3 hrs
chip antibody
RNA Pol II (Abcam, ab817: 8WG16)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
TL-Om1 cells were cross-linked with formaldehyde for 10 min. Lysates were clarified from sonicated nuclei and RNA Pol II-DNA complexes were isolated with antibody. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. Library construction and sequencing by Illumina Hiseq 4000 were performed by BGI Tech Solutions Co., Limited (Hongkong).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
21850613
Reads aligned (%)
95.6
Duplicates removed (%)
7.6
Number of peaks
5195 (qval < 1E-05)

hg19

Number of total reads
21850613
Reads aligned (%)
95.0
Duplicates removed (%)
8.5
Number of peaks
5157 (qval < 1E-05)

Base call quality data from DBCLS SRA