GSM2281662: RNAPol2 TL-Om1 DMSO ChipSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Blood
Cell type
TL-Om1
NA
NA
Attributes by original data submitter
Sample
source_name
TL-Om1 cells
cell line
TL-Om1
cell type
adult T-cell leukemia/lymphoma (ATL) cell line
treated with
control DMSO for 3 hrs
chip antibody
RNA Pol II (Abcam, ab817: 8WG16)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
TL-Om1 cells were cross-linked with formaldehyde for 10 min. Lysates were clarified from sonicated nuclei and RNA Pol II-DNA complexes were isolated with antibody. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. Library construction and sequencing by Illumina Hiseq 4000 were performed by BGI Tech Solutions Co., Limited (Hongkong).