Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Adult T-cell leukemia-lymphoma
NA
NA

Attributes by original data submitter

Sample

source_name
Primary ATL cells (ATL4)
sample id
ATL4
cell type
Primary leukemia cells derived from ATL patients
chip antibody
anti-H3K27ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
TL-Om1 cells and primary ATL cells were cross-linked with formaldehyde for 10 min. Lysates were clarified from sonicated nuclei and histone H3K27Ac-DNA complexes were isolated with antibody. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. Library construction and sequencing by Illumina Hiseq 4000 were performed by BGI Tech Solutions Co., Limited (Hongkong).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
44528955
Reads aligned (%)
99.1
Duplicates removed (%)
8.0
Number of peaks
13177 (qval < 1E-05)

hg19

Number of total reads
44528955
Reads aligned (%)
98.7
Duplicates removed (%)
8.1
Number of peaks
12997 (qval < 1E-05)

Base call quality data from DBCLS SRA