Antigen

Antigen Class

Histone

Antigen

H2Bub

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

cell type

USP7 Knockout

chip antibody

H2Bub; Cell Signaling, 5546S

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA were extracted using antibodies for EPOP, H3K4me3, Elongin B, RNA Polymerase II S5, USP7 or H2Bub. Libraries were constructed of 10-50 ng of DNA following Illumina's® protocol

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

23556509

Reads aligned (%)

96.1

Duplicates removed (%)

10.5

Number of peaks

3222 (qval < 1E-05)

mm9

Number of total reads

23556509

Reads aligned (%)

95.9

Duplicates removed (%)

10.6

Number of peaks

3236 (qval < 1E-05)