Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Others

Cell type

Thyroid Gland

MeSH Description

A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.

Sample

source_name

input_DNA

genotype

PPFPThy;PtenThy-/-

treatment

pioglitazone at a concentration of 200 parts per million in irradiated Purina rodent diet 5001

age

8-10 weeks

tissue

Thyroid gland

chip antibody

none (input)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The cells were cross-linked with formaldehyde. The tissue was sheared by gauge needle, and then sonication in a Diagenode Bioruptor (Denville, NJ). the library was constructed with WaferGen Biosystems (Fremont, CA) reagent kit #400040

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

107043939

Reads aligned (%)

97.1

Duplicates removed (%)

28.3

Number of peaks

969 (qval < 1E-05)

mm9

Number of total reads

107043939

Reads aligned (%)

96.8

Duplicates removed (%)

28.2

Number of peaks

1072 (qval < 1E-05)