Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPSC derived cardiac cells
NA
NA

Attributes by original data submitter

Sample

source_name
purified cardiomyocyte WT
cell type
iPS-derived cardiomyocytes
passages
20-30
differentiation stage
Differentiation D32
chip antibody
H3K36me3 (ab9050, lot:GR114293-2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked cell pellets were lyzed, nuclei extracted, sonicated, immunoprecipitated, washed, reverse cross-link and column purified. Barcoded ChIP-seq libraries were made from ChIP DNA using the NuGen Ovation Ultralow DR or Diagenode Microplex Kit, checked for adapter artifacts by Agilent Bioanalyzer using DNA high sensitivity reagents and chips, and quantified by qPCR using an Illumina Library Quantification kit (KAPA Biosystems)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
50590813
Reads aligned (%)
100.0
Duplicates removed (%)
0.0
Number of peaks
4230 (qval < 1E-05)

hg19

Number of total reads
50590813
Reads aligned (%)
100.0
Duplicates removed (%)
0.0
Number of peaks
4258 (qval < 1E-05)

Base call quality data from DBCLS SRA