GSM2279961: 1 8 input chip; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived cardiac cells
NA
NA
Attributes by original data submitter
Sample
source_name
purified cardiomyocyte WT
cell type
iPS-derived cardiomyocytes
passages
20-30
differentiation stage
Differentiation D32
chip antibody
Input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked cell pellets were lyzed, nuclei extracted, sonicated, immunoprecipitated, washed, reverse cross-link and column purified. Barcoded ChIP-seq libraries were made from ChIP DNA using the NuGen Ovation Ultralow DR or Diagenode Microplex Kit, checked for adapter artifacts by Agilent Bioanalyzer using DNA high sensitivity reagents and chips, and quantified by qPCR using an Illumina Library Quantification kit (KAPA Biosystems)