GSM2279078: H3K27me3 TSA 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
T cell leukaemia
cell line
Jurkat
treatment
Trichostatin A (10 nM)
time of treatment
72 hrs
chip antibody
H3K27me3 (Millipore, 07-499, lot: 2194165)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform