Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Jurkat

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

T cell leukaemia

cell line

Jurkat

treatment

sham (50Hz sinus, 0,007 mT, 5' on/10' off)

time of treatment

72 hrs

chip antibody

H3K27me3 (Millipore, 07-499, lot: 2194165)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

204134336

Reads aligned (%)

91.1

Duplicates removed (%)

33.4

Number of peaks

331717 (qval < 1E-05)

hg19

Number of total reads

204134336

Reads aligned (%)

90.7

Duplicates removed (%)

34.1

Number of peaks

330240 (qval < 1E-05)