Curated Sample Data


Genome
hg19
Antigen Class
Histone
Antigen
H3K4me2
Cell type Class
Blood
Cell type
Jurkat

Cell type information


Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by Original Data Submitter


source_name
T cell leukaemia
cell line
Jurkat
treatment
Trichostatin A (10 nM)
time of treatment
72 hrs
chip antibody
H3K4me2 (Millipore, 07-030, lot:2225237)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
100140123
Reads aligned (%)
72.1
Duplicates removed (%)
33.2
Number of peaks
48806 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA