Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
T cell leukaemia
cell line
Jurkat
treatment
ELF-MF (50Hz sinus, 1 mT, 5' on/10' off)
time of treatment
72 hrs
chip antibody
H3K4me2 (Millipore, 07-030, lot:2225237)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
242689565
Reads aligned (%)
87.8
Duplicates removed (%)
61.1
Number of peaks
124360 (qval < 1E-05)

hg19

Number of total reads
242689565
Reads aligned (%)
87.5
Duplicates removed (%)
61.7
Number of peaks
123546 (qval < 1E-05)

Base call quality data from DBCLS SRA