Curated Sample Data
Genome
hg19
Antigen Class
Histone
Antigen
H3K4me2
Cell type Class
Blood
Cell type
Jurkat
Cell type information
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by Original Data Submitter
source_name
T cell leukaemia
cell line
Jurkat
treatment
none
time of treatment
0 hrs
chip antibody
H3K4me2 (Millipore, 07-030, lot:2225237)
Metadata from Sequence Read Archive
Library Description
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform
Platform Information
instrument_model
Illumina HiSeq 2000
Number of total reads
251727237
Reads aligned (%)
90.0
Duplicates removed (%)
69.1
Number of peaks
130429 (qval < 1E-05)