Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT3

Cell type

Cell type Class
Breast
Cell type
Breast cancer cells
NA
NA

Attributes by original data submitter

Sample

source_name
A139 tumor cells
tumor
Tumor A139
treatment
N/A
chip-seq antibody
anti-STAT3 (Santa Cruz Biotechnology, sc-482)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed as previously described (Reddy et al. Molecular Cell 2013). RNA-seq was performed as previously outlned (Gertz et al. 2012) Illumina Single End for ChIP-seq, Illumina Paired End for RNA-seq

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
21695372
Reads aligned (%)
90.6
Duplicates removed (%)
4.5
Number of peaks
6419 (qval < 1E-05)

hg38

Number of total reads
21695372
Reads aligned (%)
92.2
Duplicates removed (%)
3.5
Number of peaks
6444 (qval < 1E-05)

Base call quality data from DBCLS SRA