Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT3

Cell type

Cell type Class
Breast
Cell type
HCC70
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
HCC70 cells
cell line
HCC70
treatment
ethanol
chip-seq antibody
anti-STAT3 (Santa Cruz Biotechnology, sc-482)
cell type
HCC70 (Human Tnm Stage Iiia, Grade 3, Primary Ductal Carcinoma)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed as previously described (Reddy et al. Molecular Cell 2013). RNA-seq was performed as previously outlned (Gertz et al. 2012) Illumina Single End for ChIP-seq, Illumina Paired End for RNA-seq

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
39636238
Reads aligned (%)
84.2
Duplicates removed (%)
12.2
Number of peaks
66068 (qval < 1E-05)

hg38

Number of total reads
39636238
Reads aligned (%)
84.9
Duplicates removed (%)
11.8
Number of peaks
65012 (qval < 1E-05)

Base call quality data from DBCLS SRA