Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Neutrophil progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
neutrophil progenitor cells
cell type
human cord blood CD34+ cell-derived neutrophil progenitor cells
exposure conditions
equal pool of ELF-MF, sham and no exposure
time of differentiation and exposure
5 days
differentiation
100 ng/ml G-CSF
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
77539195
Reads aligned (%)
96.8
Duplicates removed (%)
12.9
Number of peaks
3540 (qval < 1E-05)

hg19

Number of total reads
77539195
Reads aligned (%)
95.4
Duplicates removed (%)
16.3
Number of peaks
2752 (qval < 1E-05)

Base call quality data from DBCLS SRA