GSM2277183: H3K27me3 t5 ELF-MF 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Blood
Cell type
Neutrophil progenitors
NA
NA
Attributes by original data submitter
Sample
source_name
neutrophil progenitor cells
cell type
human cord blood CD34+ cell-derived neutrophil progenitor cells
exposure conditions
ELF-MF (50Hz powerline, 1 mT, 5' on/10' off)
time of differentiation and exposure
5 days
differentiation
100 ng/ml G-CSF
chip antibody
H3K27me3 (Millipore, 07-499, lot: JBC1924326)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform