Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
CD34+
NA
NA

Attributes by original data submitter

Sample

source_name
human cord blood CD34+ cells
cell type
expanded human cord blood CD34+ cells
exposure conditions
no exposure
time of differentiation and exposure
0 days
differentiation
no
chip antibody
H3K27me3 (Millipore, 07-499, lot: JBC1924326)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
35366900
Reads aligned (%)
97.0
Duplicates removed (%)
12.7
Number of peaks
11870 (qval < 1E-05)

hg19

Number of total reads
35366900
Reads aligned (%)
95.8
Duplicates removed (%)
14.9
Number of peaks
11620 (qval < 1E-05)

Base call quality data from DBCLS SRA