Curated Sample Data


Genome
hg19
Antigen Class
Histone
Antigen
H3K4me2
Cell type Class
Blood
Cell type
Neutrophil progenitors

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
neutrophil progenitor cells
cell type
human cord blood CD34+ cells-derived neutrophil progenitor cells
exposure conditions
no exposure
time of differentiation and exposure
5 days
differentiation
100 ng/ml G-CSF
chip antibody
H3K4me2 (Millipore, 07-030, lot:2225237 and 2309072)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates of crosslinked cells were sonicated, clarified and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols for HiSeq platform

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
44103508
Reads aligned (%)
95.2
Duplicates removed (%)
24.2
Number of peaks
50323 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA