GSM2267691: ChIP-seq analysis of N-Myc in human HSPCs, untreated, replicate 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MYCN
Cell type
Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
bone marrow CD34+ cells, untreated
cell type
human bone marrow hematopoietic stem and progenitor cells
chip antibody
mouse monoclonal N-Myc antibody raised against N-terminal recombinant N-Myc of human origin, clone B8.4.B (Santa Cruz Biotechnology, Cat # sc-53993, Lot # K1315)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Thirty million human bone marrow CD34+ cells were fixed and used for ChIP and precipitated using 3 micrograms of antibody. Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.