Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYCN

Cell type

Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
bone marrow CD34+ cells, untreated
cell type
human bone marrow hematopoietic stem and progenitor cells
chip antibody
mouse monoclonal N-Myc antibody raised against N-terminal recombinant N-Myc of human origin, clone B8.4.B (Santa Cruz Biotechnology, Cat # sc-53993, Lot # K1315)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Thirty million human bone marrow CD34+ cells were fixed and used for ChIP and precipitated using 3 micrograms of antibody. Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
8861539
Reads aligned (%)
90.4
Duplicates removed (%)
18.3
Number of peaks
2246 (qval < 1E-05)

hg19

Number of total reads
8861539
Reads aligned (%)
90.1
Duplicates removed (%)
19.3
Number of peaks
2279 (qval < 1E-05)

Base call quality data from DBCLS SRA