Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ama-1

Cell type

Cell type Class
Embryo
Cell type
Mixed embryo
NA
NA

Attributes by original data submitter

Sample

source_name
mixed stage early embryos
range of embryonic cell stages
1cell to 350 cell stage
strain
KW2309
genotype/variation
control
chip antibody
anti-AMA-1 (Novus Biological, Cat# 38520002)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA Pol II-DNA complexes were isolated using AMA-1 antibody from prepared lysates. DNA CHIP-seq library is prepared as described in Bowmen et al (doi:10.1186/1471-2164-14-466)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce10

Number of total reads
17446004
Reads aligned (%)
148.3
Duplicates removed (%)
23.7
Number of peaks
4759 (qval < 1E-05)

ce11

Number of total reads
17446004
Reads aligned (%)
148.3
Duplicates removed (%)
23.7
Number of peaks
4759 (qval < 1E-05)

Base call quality data from DBCLS SRA