Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Su(var)205

Cell type

Cell type Class
Larvae
Cell type
3rd instar
NA
NA

Attributes by original data submitter

Sample

source_name
whole_larvae_nuclei_isolation
developmental stage
3rd instar larvae
genotype/variation
K9R
chip antibody
anti-HP1a (Covance, PRB-291C, Lot #D14JF02175)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated from 50 whole larvae per replicate and fixed in 4% formaldehyde for 20 minutes. Chromatin was sonicated to 200-300bp fragments. 2.5% of Drosophila virilis chromatin from 3rd instar larvae was added as a spike-in. 10% of extract was set aside as an input. Pull down was performed with protein A/G magnetic beads preincubated with anti-HP1a PRB-291C (Covance). ThruPlex DNA Seq Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
15423880
Reads aligned (%)
91.7
Duplicates removed (%)
4.8
Number of peaks
0 (qval < 1E-05)

dm6

Number of total reads
15423880
Reads aligned (%)
89.9
Duplicates removed (%)
6.5
Number of peaks
1293 (qval < 1E-05)

Base call quality data from DBCLS SRA