GSM2266286: HBE EHF ChIP-seq replicate 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EHF
Cell type
Cell type Class
Lung
Cell type
NHBE
Tissue
epithelium
Lineage
endoderm
Description
bronchial epithelial cells
Attributes by original data submitter
Sample
source_name
EHF ChIP-seq
cell line
HBE cells
cell type
Primary human bronchial epithelial cells
chip antibody
EHF (Clone 5A.5)
input used for homer peak calling
HBE input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed in lysis buffer (5mM PIPEs pH 8.0, 85mM KCL, 0.5% NP-40, 1x Protese Inhibitor Cocktail (Roche)). Nuclear preparation was isolated by centrifugation, lysed in RIPA buffer (1x PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, Protease Inhibitor Cocktail), and sonicated. Protein-DNA complexes were isolated with antibody. DNA ends were blunted using T4 DNA polymerase, Klenow DNA polymerase, and T4 polynucleotide kinase. DNA was then incubated with Klenow exo- to add 5’ adenine overhangs. Multiplex adaptors were ligated to the ends and converted to dsDNA using 5 cycles of PCR. DNA was size-selected to contain fragments between 200 and 300bp and PCR amplified for 5 cycles. DNA was purified with 1.2x AMPusreXP beads. Sequencing was performed on an Illumina Hi-Seq.