Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Neural
Cell type
SF8628
NA
NA

Attributes by original data submitter

Sample

source_name
SF8628 (H3.3K27M)
cell type
Diffuse Intrinsic Pontine Glioma (DIPG)
passages
Low passages (6-10)
chip antibody
EZH2 (CST, 5246)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted using QIAGEN RNeasy kit. DNA after ChIP protocol was isolated using QIAGEN PCR purification kit. ChIP-sequencing libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250- 450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit. RNA-sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA Preparation Kit with Ribo-Depletion. Input RNA quality was validated using the Agilent RNA 6000 Nano Kit. 200ng-1 µg of total RNA was used as starting material. Libraries were validated using the Agilent DNA 1000 Kit

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
19773026
Reads aligned (%)
91.0
Duplicates removed (%)
7.0
Number of peaks
812 (qval < 1E-05)

hg38

Number of total reads
19773026
Reads aligned (%)
92.3
Duplicates removed (%)
6.2
Number of peaks
750 (qval < 1E-05)

Base call quality data from DBCLS SRA