Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MEN1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 breast cancer cells
chip antibody
anti-Menin(MEN1) (A300-115A,Bethyl)
cell line
MCF7
treatment
10 nM estradiol (E2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, lysed in a buffer containing sarkosyl and sonicated. ThruPLEX-FD Prep kit, Rubicon Genomics

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
55753777
Reads aligned (%)
68.6
Duplicates removed (%)
11.9
Number of peaks
6127 (qval < 1E-05)

hg38

Number of total reads
55753777
Reads aligned (%)
70.1
Duplicates removed (%)
10.9
Number of peaks
6579 (qval < 1E-05)

Base call quality data from DBCLS SRA