Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MEN1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 breast cancer cells
chip antibody
anti-Menin(MEN1) (A300-115A,Bethyl)
cell line
MCF7
treatment
vehicle

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, lysed in a buffer containing sarkosyl and sonicated. ThruPLEX-FD Prep kit, Rubicon Genomics

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
40216708
Reads aligned (%)
72.2
Duplicates removed (%)
7.6
Number of peaks
4165 (qval < 1E-05)

hg38

Number of total reads
40216708
Reads aligned (%)
73.8
Duplicates removed (%)
6.7
Number of peaks
4410 (qval < 1E-05)

Base call quality data from DBCLS SRA