Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
Drosophila S2 cells
cell line
S2
treatment
untreated
chip antibody
H3K27ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Pol II ChIP material was prepared as described previously (Muse et al., 2007). Immunoprecipitated material was purified using the Qiaquick PCR purification kit

Sequencing Platform

instrument_model
NextSeq 500

dm6

Number of total reads
94859149
Reads aligned (%)
96.1
Duplicates removed (%)
43.0
Number of peaks
0 (qval < 1E-05)

dm3

Number of total reads
94859149
Reads aligned (%)
96.3
Duplicates removed (%)
42.4
Number of peaks
7385 (qval < 1E-05)

Base call quality data from DBCLS SRA