Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF-7

biomaterial_provider

ATCC

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

MCF-7

cell organism

human

cell description

mammary gland, adenocarcinoma. (PMID: 4357757), newly promoted to tier 2: not in 2011 analysis

cell karyotype

cancer

cell lineage

ectoderm

cell sex

F

antibody

CTCF

antibody antibodydescription

Rabbit polyclonal. Antibody Target: CTCF

antibody targetdescription

CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances.

antibody vendorname

Millipore

antibody vendorid

07-729

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000968

labexpid

DS16199

labversion

Hotspot-v5.2,lmax-v1.0,WindowDensity-bin20-win+/-75

replicate

2

Sequenced DNA Library

library_name

GSM1022663: UW_ChipSeq_MCF-7_CTCFRep2

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

32412076

Reads aligned (%)

74.7

Duplicates removed (%)

6.7

Number of peaks

44708 (qval < 1E-05)

hg19

Number of total reads

32412076

Reads aligned (%)

74.2

Duplicates removed (%)

8.0

Number of peaks

44670 (qval < 1E-05)