Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H7
NA
NA

Attributes by original data submitter

Sample

source_name
H7-hESC
biomaterial_provider
WiCell Research Institute
lab
UW
lab description
Stamatoyannopoulous - University of Washington
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
H7-hESC
cell organism
human
cell description
undifferentiated embryonic stem cells
cell lineage
inner cell mass
cell sex
U
antibody
Input
antibody description
Control signal which may be subtracted from experimental raw signal before peaks are called.
treatment
diffProtA_14d
treatment description
H7 embryoid bodies differentiation protocol for cardiomyocyte, endothelial, smooth muscle, with markers cardiac troponin T (cTnT), CD31/PECAM1, smooth muscle alpha actin (SMA), respectively, using proteins from bone morphogenic protein 4 (BMP4), activin A and basic fibroblast growth factor (bFGF) for 14 days, cell sorting and specific growth factors were used, lineage: mesoderm
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH002532
labexpid
DS14111
labversion
WindowDensity-bin20-win+/-75
replicate
1

Sequenced DNA Library

library_name
GSM1022649: UW_ChipSeq_H7-hESC_Input_diffProtA_14dRep1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
27085975
Reads aligned (%)
87.7
Duplicates removed (%)
3.2
Number of peaks
486 (qval < 1E-05)

hg19

Number of total reads
27085975
Reads aligned (%)
87.2
Duplicates removed (%)
4.0
Number of peaks
674 (qval < 1E-05)

Base call quality data from DBCLS SRA