Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Blood

Cell type

NB-4

Primary Tissue

Blood

Site of Extraction

Bone Marrow

Tissue Diagnosis

Leukemia

Sample

source_name

NB4

biomaterial_provider

Refer to protocol documents for differing sources, UW: DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) ACC-207 http://www.dsmz.de/ or Yale (Stanford): National Cell Culture Center http://www.nccc.com/

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

NB4

cell organism

human

cell description

acute promyelocytic leukemia cell line. (PMID: 1995093)

cell karyotype

cancer

cell lineage

mesoderm

cell sex

U

antibody

CTCF

antibody antibodydescription

Rabbit polyclonal. Antibody Target: CTCF

antibody targetdescription

CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances.

antibody vendorname

Millipore

antibody vendorid

07-729

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000970

labexpid

DS16345

labversion

Hotspot-v5.2,lmax-v1.0,WindowDensity-bin20-win+/-75

replicate

1

Sequenced DNA Library

library_name

GSM1022643: UW_ChipSeq_NB4_CTCFRep1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

26228839

Reads aligned (%)

85.8

Duplicates removed (%)

6.8

Number of peaks

37205 (qval < 1E-05)

hg19

Number of total reads

26228839

Reads aligned (%)

85.1

Duplicates removed (%)

8.2

Number of peaks

37109 (qval < 1E-05)