Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Muscle
Cell type
Rhabdomyosarcoma
MeSH Description
A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9)

Attributes by original data submitter

Sample

source_name
RMS cell line, mutant HRAS
antibody
EZH2, Active motif (cat39901 lot 27210001 pAb)
enrichment target
EZH2
target function
Polycomb Complex
time
48 hr
treatment
Trametinib 100nM

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation ChIP-seq and DNase-seq

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
29083038
Reads aligned (%)
81.3
Duplicates removed (%)
16.4
Number of peaks
8567 (qval < 1E-05)

hg38

Number of total reads
29083038
Reads aligned (%)
82.3
Duplicates removed (%)
15.8
Number of peaks
8663 (qval < 1E-05)

Base call quality data from DBCLS SRA