Curated Sample Data


Genome
hg19
Antigen Class
TFs and others
Antigen
MAPK1
Cell type Class
Muscle
Cell type
Rhabdomyosarcoma

Cell type information


MeSH Description
A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9)

Attributes by Original Data Submitter


source_name
RMS cell line, mutant HRAS
antibody
anti-MAP Kinase 2, Millipore (cat 05-0157, lot 2654214 mAb)
enrichment target
ERKmp
target function
Nuclear Kinase
time
48 hr
treatment
DMSO

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation ChIP-seq and DNase-seq

Platform Information


instrument_model
NextSeq 500

External Database Query

Logs in read processing pipeline


Number of total reads
29868971
Reads aligned (%)
95.7
Duplicates removed (%)
13.5
Number of peaks
1492 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA