Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAPK1

Cell type

Cell type Class
Muscle
Cell type
Rhabdomyosarcoma
MeSH Description
A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9)

Attributes by original data submitter

Sample

source_name
RMS cell line, mutant HRAS
antibody
anti-MAP Kinase 2, Millipore (cat 05-0157, lot 2654214 mAb)
enrichment target
ERKmp
target function
Nuclear Kinase
time
48 hr
treatment
DMSO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation ChIP-seq and DNase-seq

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
29868971
Reads aligned (%)
82.8
Duplicates removed (%)
13.5
Number of peaks
1492 (qval < 1E-05)

hg38

Number of total reads
29868971
Reads aligned (%)
83.7
Duplicates removed (%)
12.9
Number of peaks
1533 (qval < 1E-05)

Base call quality data from DBCLS SRA