Antigen

Antigen Class

Histone

Antigen

H2BK120ub1

Cell type

Cell type Class

Breast

Cell type

MDA-MB-436

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MB436 cells, H2BK120ub1 ChIP

cell line

MB436

cell type

Breast cancer cell line

treatment

Control

chip antibody

H2BK120ub1 (Millipore, 05-1312, lot 2128268)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

50321644

Reads aligned (%)

92.4

Duplicates removed (%)

4.0

Number of peaks

500 (qval < 1E-05)

hg19

Number of total reads

50321644

Reads aligned (%)

91.7

Duplicates removed (%)

4.6

Number of peaks

417 (qval < 1E-05)