Antigen

Antigen Class

Histone

Antigen

H3K79me2

Cell type

Cell type Class

Breast

Cell type

MDA-MB-468

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MB468 cells, H3K79me2 ChIP

cell line

MB468

cell type

Breast cancer cell line

treatment

Control

chip antibody

H3K79me2 (Abcam, ab3594)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

60698982

Reads aligned (%)

99.0

Duplicates removed (%)

5.9

Number of peaks

9495 (qval < 1E-05)

hg19

Number of total reads

60698982

Reads aligned (%)

98.7

Duplicates removed (%)

6.3

Number of peaks

9417 (qval < 1E-05)